Expression of homologous RND efflux pump genes is dependent upon AcrB expression: implications for efflux and virulence inhibitor design
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چکیده
منابع مشابه
Expression of homologous RND efflux pump genes is dependent upon AcrB expression: implications for efflux and virulence inhibitor design
OBJECTIVES Enterobacteriaceae have multiple efflux pumps that confer intrinsic resistance to antibiotics. AcrB mediates clinically relevant multidrug resistance and is required for virulence and biofilm formation, making it an attractive target for the design of inhibitors. The aim of this study was to assess the viability of single transporters as a target for efflux inhibition using Salmonell...
متن کاملSite-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB.
The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while other targeted mutations, including conversion of phenylalanines 136, 178, 615, 617, and 628 to a...
متن کاملEffects of synthesized iron oxide nanoparticles from Ziziphora clinopodioides on expression of the efflux pump genes of Staphylococcus aureus
Introduction: One of the important factors causing nosocomial infections is drug-resistant Staphylococcus aureus strain, so correspondingly, having norA efflux pump is one of the bacterial's ability to generate drug resistance. Iron has been used as a nanoparticle with antimicrobial activity against bacteria. The aim of this study was to investigate the effect of iron oxide nanoparticles synthe...
متن کاملبررسی مقاومت آنتی بیوتیکی ایزوله های کلبسیلاپنومونیه و میزان بیان ژنهای Real-Time PCR بااستفاده ازacrA وoqxA
Background: The increasing emergence of antimicrobial resistance among gram negative bacteria especially Enterobacteriaceae has become a global problem. Klebsiella pneumoniae is an opportunistic pathogen, which recently due to causing broad spectrum of disease and antibiotic resistance has been lionized. Efflux pumps are one of the antibiotic resistance mechanisms, which were introduced in t...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2014
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/dku380